CARBOXYMETHYL TAMARIND KERNEL GUM/CUO-BASED NANOCOMPOSITE HYDROGEL IN ORAL DRUG DELIVERY OF CIPROFLOXACIN

Abstract

Carboxymethyl tamarind kernel gum (CMTKG), bovine serum albumin (BSA), acrylamide (AAM) and Acrylic acid (AA) was combined to create a novel hydrogel system that included ciprofloxacin (CFX) as a model therapeutic agent. This study investigates CMTKG-g poly(acrylamide-co-acrylic acid)/BSA hydrogel containing Phytogenic CuO nanoparticles for drug delivery of Ciprofloxacin. The free radical graft polymerization method is used to graft acrylamide and acrylic acid onto the Carboxymethyl tamarind kernel gum backbone. Lysine coated CuO nanoparticles were synthesized using plant extract of Madagascar periwinkle that follows green synthesis. Various hydrogel compositions were synthesized with different compositions of Bovine serum albumin and nanoparticles. Swelling studies and drug release studies were conducted carefully at pH 2.2 and 7.4. According to swelling studies, it is observed that the hydrogel swelled more at pH 7.4 and less at pH 2.2. The Equilibrium swelling ratio of nanocomposite hydrogel was found to be (306-634) % in pH 2.2 and (1096-1210) % in pH 7.4. Subsequent examination of drug release revealed higher release at pH 2.2. Nanocomposite hydrogel shows drug release 59-62% in pH 2.2 and 40-50.5% in pH 7.4 in 7h. The Korsmeyer–Peppas model was found to be the best fit for the drug release profile based on kinetic evaluations. We added BSA and NPs to reduce the swelling and enhance the cross linking in hydrogel network to overcome the burst release of the Ciprofloxacin and facilitate the controlled release of the drug. Hydrogels were characterized by following characterization techniques for the studies which are FTIR, PXRD, FESEM, Zeta Potential, Antimicrobial activity and MTT Assay. The particle size was found to be 26.23±2.95 using Debye-Scherrer equation. At a concentration of 80 μg/mL, antimicrobial assays showed significant inhibition. Inhibition zones of 10 mm against E. coli and 11 mm against S. aureus were shown by the Ciprofloxacin-loaded nanocomposite hydrogel. MTT assay was performed at 50-250 μg/mL concentration on L929 cell lines. The hydrogel’s outstanding biocompatibility was confirmed that showed >90% cell viability. As a result, this hydrogel formulation shows great promise for improved cellular compatibility, bactericidal efficacy, and regulated drug delivery.