COMPARATIVE GENOMICS ANALYSIS OF ANTIBIOTIC RESISTANCE GENES IN ALCALIGENES FAECALIS

Abstract

Alcaligenes faecalis, opportunistic gram-negative bacteria which has a rod shape structure. It is associated with raising concern of Antibiotic Resistance, which is responsible of causing infections among Humans. This study presents a in-depth comparative genomics analysis of 24 complete genome of Alcaligenes faecalis retrieved from NCBI. Genome annotation conducted through the RAST Server, leading to Phylogenetic analysis of 24 strains. Phylogenetic tree which reveals their evolutionary relationship among themselves. Resistome profile has built using database like CARD and Resfinder, shows Antibiotic resistance gene presence among those strains. Pan genome analysis has performed using Roary uncovered 1907 conserved core genes, 9612 acquired accessory genes, also resulted in quantitative ARGs frequency helped to classify gene into core and accessory. ARGs were mapped on Pan genome revealed presence on ARGs on core genes and Accessory genes. Phylogenetic comparison between core gene and accessory gene allowing discrimination between lineage-based divergence and traits acquired from Horizontal gene transfer. Strains were categorized based on their isolation source shows A. faecalis is widely distributed in Environment, also revealed host associated strains. This study investigated phylogenetic relationship and distribution of the genes responsible for Antibiotic resistance, involving in adaptation distribution and in pan genome analysis.