IDENTIFICATION OF PHYTOCHEMICALS IN PLANTS WITH POTENTIAL ANTI- DIABETIC ACTIVITY USING IN-SILICO TECHNIQUES

Abstract

Background: The prevalence of diabetic person and the negative consequence of readily accessible anti-hyperglycemic medications have drawn researchers' immersion to the need for novel therapeutic strategies. One method for reducing postprandial hyperglycemia by postponing the absorption and digestion of crabs is to inhibit the activity of enzymes that dissolve carbohydrates. The identification of phytochemicals from plants with potential anti-diabetic activity using in- silico techniques has gained significant attention in recent years. The telltale sign of diabetes is high blood glucose levels., a chronic metabolic illness, and natural substances derived from plants are showing promising outcomes in treating this condition. This abstract makes an effort to provide a summary of the research conducted in this area. Researchers have discovered phytochemicals that show potential interactions with enzymes and receptors linked to obesity, including dipeptidyl peptidase is IV (DPP-IV), protein the tyros phosphate 41B (PTP1B), - glucosidase, the peroxisome proliferator-activated receptor (PPAR) gamma (PPAR), and 11- hydroxysteroid dehydrogenase type 1 (11-HSD1). Numerous investigations have found phytochemicals with strong binding affinities to various molecular targets, demonstrating their potential as anti-diabetic medicines. Objectives: Investigating phytochemicals, antioxidants, the inhibition of digestive enzymes, and the molecular docking of powerful extracts were among the goals of the study. Materials and Procedures In this work, we evaluate the total phenolic and avonoids concentrations as well as The inhibitory effects of A. racemosus, B. ciliata, C. gigantea, M. pudica, P. demblica, and S. nigrum on substrate-based -glucosidase and -amylase. The DPPH radical's scavenging was another way to measure antioxidant activity. Agar well diffusion method was also used to study antibacterial activity. Bioactive substances from B. ciliatag were molecularly docked using AutoDock vina. Results: B. ciliata, M. pudica, and P. emblica show signicant impede properties against the α- glucosidase and α-amylase in IC50 (μg/ml) of (1.24 ± 0.01,45.19 ±1.06), (34.73 ± 0.64,99.93 ± 0.9) nossignicant activity) accordingly suggesting an excellent source fordisolating a possible candidate for a drug for diabetes. In addition to significant antibacterial activity against the vi bacteria Staphylococcus aureus and Klebsiella, pneumonia, these plants additionally with IC50 values ranging from 12.2 to 25.5 g/mL, demonstrated substantial antioxidant activity.Bergeniag looked to be a powerful amylase and glucosidase inhibitor. It is necessary to conduct more study to characterize inhibitory substances. Conclusion: Bergenia extract proved to be a strong inhibitor of both glucosidase and amylase. It is necessary to conduct more study to characterize inhibitor substances.