BIOLOGICAL ACTIVITIES AND PHYTOCHEMICAL ANALYSIS OF TWO DIFFERENT FRUITS OF MURRAYA KOENIGII

Abstract

Murraya koenigii also known as curry leaves have been known for various traditional medicinal practices. This plant belongs to Rutaceae family. The purpose of this present study is examining the phytochemicals and biological profiling of methanolic extract of two different shaped, oval and round fruits of Murraya koenigii plant. The bioactive constituents were investigated using GC-MS. GC-MS determined the presence of pyranone (13.42%) and cis-vaccenic acid (9.68%) in the methanolic extract of oval and round fruits of Murraya koenigii. UHPLC-QTOF-MS analysis confirmed the existence of 50 secondary metabolites in the methanolic extract of oval fruit and 26 secondary metabolites in round fruits. These secondary metabolites include, Fosfomycin calcium, 3-Methyl-5-(trifluoromethyl)pyrazole, Dansylamide, N-glycylacrylamide, Thiodan sulfate, Biindoline and so on. In biological activities, extract of both the fruits of Murraya koenigii were analysed for antioxidant, antifungal, antiacne and antidiabetic activity. Among the methanolic extract of the two samples, round fruit showed better antioxidant activity than oval fruit sample. In case of antifungal activity of Murraya koenigii fruits, round fruits revealed slightly better activity towards C. Albicans in comparison to oval fruits. Oval shaped fruits showed higher anti diabetic activity than the round shaped fruits. Additionally, the round shape fruits were most effective against Propionibacterium acnes. Overall, this study suggests that Murraya koenigii fruits have the potential to be used as a new medicinal food and can provide a new aspect to develop more potent therapeutics. vi GRAPHICAL ABSTRACT After collection of round and oval fruits, they were washed and cleaned separately. Methanolic extract was prepared of these fruits. Further, they were filtered and concentrated via rotary evaporator. The obtained extracts showed difference in colour. These extracts were used to evaluate the phytochemicals and biological profiling.