CuS@rGO GRAFTED-PEDOT:PSS PAPER BASED ELECTROCHEMICAL BIOSENSOR FOR FENITROTHION DETECTION

Abstract

This study described a novel paper-based biosensor that can quickly and accurately detect fenitrothion pesticides. This work uses CuS@rGO grafted-PEDOT:PSS based paper as a transducer. Strong non-covalent interactions between PEDOT:PSS and the paper's cellulose molecules cause the polymer to alter its conformation, which significantly increases conductivity. To improve the electrochemical characteristics, CuS@rGO/PEDOT:PSS/WP electrodes were further doped with a variety of organic solvents, including methanol, ethylene glycol (EG), dimethyl sulfoxide (DMSO), N, N-dimethyl formamide (DMF), and N-methyl pyrrolidone (NMP). The conducting paper doped with DMSO is determined to have the highest conductivity out of all of these solvents. Then, using glutaraldehyde as a cross-linking agent, the AChE enzyme is attached onto the modified conducting paper strips to speed up the specific identification of fenitrothion. Under a physiological range of 1-80 pM for fenitrothion, electrochemical tests have demonstrated the high sensitivity and low detection limit of this conducting paper-based electrode, which are 0.505 mA/pM and 0.28 pM, respectively. The ITO, gold, and glassy carbon electrodes are known to have limited applications due to their high cost, fragility, limited flexibility, and environmental problems. This paper electrode, on the other hand, may be a very promising substitute. The accuracy of the suggested biosensor was thoroughly assessed in two genuine samples rice and tomato improving its effectiveness for fenitrothion (FNT) detection in real-world applications.