GRAPHITIC CARBON NITRIDE DECORATED MOLYBDENUM SULFIDE-BASED ELECTROVHEMICAL BIOSENSOR FOR TRICHLORFON DETECTION

Abstract

Organophosphorus pesticides as insecticides are extensively used in agricultural products to enhance agricultural production. Organophosphate pesticides instigate toxicity by inhibiting the function of the AChE enzyme and finally affecting the central nervous system. In recent years, acetylcholinesterase (AChE) biosensors have emerged as highly sensitive tools for the detection of toxicity in pesticides. These biosensors can replace classical methods owing to their high selectivity and quick response. Here, graphitic carbon nitride (g-C3N4) has been strengthened with molybdenum disulfide (MoS2) to create an electrochemical biosensor for the detection of trichlorfon. AChE enzymes were covalently attached to the g-C3N4@MoS2 composite integrated electrodes, and potentiometric response was measured using differential pulse voltammetry. The developed biosensors exhibit enhanced electrochemical sensing performance and increased electrocatalytic properties due to the combined action of g C3N4@MoS2. By utilizing glutaraldehyde as a cross-linking agent to immobilize acetylcholinesterase (AChE) on the g-C3N4@MoS2 film, a responsive biosensor is developed via electrophoretic deposition of g-C3N4@MoS2 on the surface of ITO substrate. The produced biosensor performed admirably because g-C3N4 improves electron mobility by forming a 2D– 2D hybrid interface with MoS2 nanosheets. The fabricated biosensor exhibits a low detection limit of 2.1 nM with a broad linear range of 5-100 nM.