A GREEN APPROACH FOR THE SYNTHESIS OF STABLE SILVER NANOPARTICLES USING KALANCHOE BLOSSFELDIANA AND THEIR CHARACTERIZATION

Abstract

A green approach for the synthesis of nanoparticles has attracted significant attention owing to its advantages including single-step synthesis process, rapidity, environmental friendliness, and cost-efficiency. The genus Kalanchoe has a rich application in medicine, attributed to its notable therapeutic properties. Various species within this genus share both chemical and physical traits. This study focuses on a particular species of Kalanchoe-Kalanchoe blossfeldiana. Stable silver nanoparticles (AgNPs) were synthesized through a green approach using the Kalanchoe blossfeldiana extract as a reducing agent. The synthesized AgNPs underwent various characterization to study their physical, chemical as well as biological properties. They were confirmed through multiple techniques including UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The XRD peaks obtained confirm the crystalline nature of the biosynthesized silver nanoparticles. The crystallite size of the synthesised silver nanoparticle is 18.02nm. The surface charge potential of the biosynthesized AgNPs is –12.6 mV showing good stability with average particle size is around 29.6 nm. Thus, stable AgNPs of different sizes range from 10 to 35 nm were formed. The absorption spectra were recorded with wavelengths ranging from 280 to 700 nm. The AgNPs showed their maximum absorption peak at around 451 nm. The average size of the synthesized AgNPs is 21 nm.