SYNTHESIS AND BIOPHYSICAL CHARACTERIZATION OF IMIDAZOLE AND DEHYDROPEPTIDE BASED SELF-ASSEMBLED NANOSTRUCTURES

Abstract

The study focuses on the synthesis and characterization of imidazole-containing dehydropeptide nanoparticles through solution and solid-phase peptide synthesis. In pharmaceutical chemistry, imidazole and its derivatives are among the most significant and often utilized heterocycles. Due to the distinct structural characteristics of the imidazole ring, imidazole derivatives can bind to a variety of enzymes and receptors with ease through a variety of weak interactions, exhibiting a broad spectrum of biological and pharmacological effects. Many imidazole derivatives have been utilized extensively as anti-inflammatory, anticancer, antiviral, antibacterial, and other therapeutic agents in the field of medicinal chemistry. Therefore, in this study, the synthesized nanostructures will be evaluated for the anti-microbial properties in Gram-positive and Gram negative bacteria through Zone of Inhibition (ZOI) and Minimum Inhibitory Concentration (MIC). The physical characterization of the subjected nanoparticles was done using various spectroscopic techniques, including Infrared (IR), Nuclear Magnetic Resonance (NMR), and Ultraviolet (UV) spectroscopy, and size and zeta-potential analysis of the nanoparticles were carried out by DLS. It was observed that the synthesized peptide was self-assembled in aqueous media in the range of 222-650 nm with a zeta potential ranging from (+4) to (+9). Furthermore, it was observed that the projected nanostructures showcased initial antimicrobial activity, thereby contributing to potential applications in the field of nanomedicine.