INDENTIFICATION OF DIFFERENTIALLY REGULATED GENES BETWEEN NORMAL AND TNF INDUCED IN HUMAN TRANSCRIPTOME

Abstract

According to Human Genome Project humans contains nearly 25,000 genes. Humans differs from each other just because of 0.1% of DNA. Since all the cells of an individual contains similar genetic material still they differ among one another in function and this is because of the differential gene expression. Since cells respond differently to different stimulus it is interesting to note their response. Gene expression is the main reason how cell gone a respond to different stimuli. In this study we have taken sequenced mRNA from human cell lines which was treated with TNF-alpha for some time period and try to identify all the differential expressed genes using RNA-Seq. We will extend our study to find out the pathways in which these genes have involved. In this study we have used DESeq2 package for normalization, statistical analysis and for visualization of dataset. Genes obtained at the end of the analysis can be act as biomarkers for the cancer treatment.