SCREENING, ISOLATION & OPTIMIZATION FOR ACTIVITY OF AMYLASE PRODUCTION BACTERIA FROM SOIL SAMPLE

Abstract

The amylases from the bacterial sources are the among them, belongs to the genus bacillus have been studies deliberately for their commercial utilization. The output of the amylase functioning on the starch are known as Dextrins (alpha-1,4 linked glucose dimmers such as Maltose) Alpha-linked glucose trimers (Maltotoris) and branched oligosaccharides of 6-8 glucose subunits that carries both alpha -1,6 and alpha-1,4 linkages. At present, amylase producing bacteria are being isolated and are then characterized for their morphological and biochemical properties. The major objective to isolate & screen the amylase-secreting bacteria from abundant soil samples is to determine the hinderance to pathogenic bacteria. This study has been aimed to extract the various strains of Amylase, their screening and identification. The isolated bacteria will be gram-positive from different bacterial strains. The specifics amylase activity will be tracked to check-out the enzyme kinetics and the results will be shown at the highest activity after the sequential incubation of various strains and thus incubation will be taken for the further analysis. This amylase-producing bacterial strains posses number of industrial applications in fruit, agricultural, medicinal industry.