SYNTHESIS OF NANO - POLYANILINE FILM BY ELECTROCHEMICAL POLYMERIZATION AND ITS APPPLICATION IN GLUCOSE SENSING

Abstract

Biosensors have ubiquitous biomedical applications that cover a wide range of areas such as diagnosis, point-of-care monitoring of treatment and disease progression, environmental monitoring, food quality control, drug delivery, drug discovery, forensics and other biomedical research. Biosensors may indeed be developed using a diverse range of methodologies. Their interactions with high-affinity biomolecules facilitates sensitive and selective detection of a plethora of analytes. The use of conducting polymers in sensing is intriguing since they are known to improve the sensitivity of a biosensor. Polyaniline (PANI) is a notable conductive polymer, and it has gained considerable attention from researchers in the field of nanotechnology for the enhancement of sensors. Because of its ease of synthesis and outstanding environmental stability, PANI is readily doped by various acids and dopants. PANI is a highly conductive polymer with distinct qualities such as easy synthesis, low cost, and good environmental resilience. PANI's unique features have led to its widespread use in a variety of applications such as drug delivery, anti-corrosion materials, scaffold and sensing devices manufacturing. In this present thesis, polyaniline (PANI) was fabricated over ITO coated glass slides and further composite electrodes were fabricated by immobilization of Glucose oxidase (GOx) into electrochemically polymerized polyaniline thin films for biosensor application. The electrochemical polymerization of PANI on ITO was performed in aqueous solution of 0.1M HCl and 0.3 M aniline at constant current of 0.15 A. Furthermore, GOx enzyme combined with HRP was immobilized on the Polyaniline fabricated electrode. Analytical characterization of GOx modified polyaniline electrode in comparison to polyaniline electrode and bare ITO were carried out by Cyclic voltammetry and Amperometric curves for different glucose concentrations ranging from 50mM to 200mM were obtained.