IN SILICO IDENTIFICATION OF COMMON PROTEINS INVOLVED IN CROTONYLATION AND ACETYLATION IN ALZHEIMER'S DISEASE AND DRUG REPURPOAING

Abstract

Dementia is one of leading cause of the most common neuronal disorders Alzheimer’s Disease (AD) that remains untreated even after decades of research. Post-translational modification (PTMs) plays many roles in protein turnover rate accumulation of aggregate and can also help in the degradation of disease-causing toxic metabolites. In this study we tried to understand the involvement of Crotonylation and acetylation in AD by using computational tools and database and finally identify a possible drug for treatment using Drug Repositioning tools. Through extensive literature analysis we found that P300 and CBP are the common enzymes associated with protein crotonylation and acetylation in Alzheimer’s Disease (AD) proving the association between these two PTMs. Using step by step computational analysis we found hub genes associated with the PTMs and AD. With the help of a comprehensive visual drug network gene analysis tool, we identified Arsenic Trioxide as potential drug which interacted with the MAPK1, JUN and MAPK3 genes associated with AD. And finally using DrugNet website tool for drug disease association and PhospNet tool for disease-gene association studies developed by group of researchers from university of Granada, Spain we validated the results and concluded that Arsenic Trioxide is associated with different form of Dementia including Frontotemporal dementia, vascular Dementia and Dementia associated with lewy bodies.