BIOSYNTHESIZED RGO@ZNO NANOCOMPOSITE FOR BOVINE SERUM ALBUMIN DETECTION

Abstract

Bovine serum albumin (BSA) is a globular protein that plays a pivotal role in the delivery of fatty acid/amino acid. The presence of multiple binding sites in BSA allows it to interact with many inorganic, organic molecules, ionic metals, and radicals. The reduction of BSA may cause various diseases in cattle families, thus making the quantification of BSA crucial. In the present work, efforts have been made to fabricate an immunosensor for the detection of BSA using reduced graphene oxide@zinc oxide nanocomposite (rGO@ZnO). The nanocomposite has been synthesized via a biosynthetic approach, where Aloe vera leaf extract was used as a reducing agent. The characterization of the nanocomposite was conducted using UV-visible spectroscopy, FT-IR, Zeta Potential and TGA techniques. Further, the rGO@ZnO nanocomposite was deposited electrophoretically on indium tin oxide coated glass substrate, followed by the immobilization of antibodies. The fabricated rGO@ZnO biosensor exhibits a good linear range (0.001 to 30 ng mL-1 ), low detection limit with acceptable selectivity and stability.