DEVELOPMENT OF Fe-MoO3 NANOCOMPOSITE BASED POINT OF CARE DEVICE FOR BREAST CANCER DETECTION

Abstract

In this work, a new Fe- MoO3 nanocomposite ITO based electrochemical biosensor is developed, as a robust, sensitive and low-cost point -of-care device for breast cancer detection, using CYFRA21-1 as a biomarker. The Fe-MoO3 nanocomposite was synthesized by Hydrothermal method, and the Fe content in the nanocomposite was optimized. X-Ray Diffraction (XRD) and scanning electron microscopy (SEM) techniques were used for phase identification and surface morphology analysis of synthesized nanocomposite.( Fe-MoO3). The 3-aminopropyl triethoxysilane (APTES) was used for the functionalization of Fe-MoO3, and electrophoretic deposition (EPD) technique was used for deposing it onto ITO coated glass electrode. CYFRA21-1 antibody was immobilized using EDC-NHS, and Bovine serum albumin (BSA) was used to block the non specific active sites. For the immunoelectrode ( BSA/anti-CYFRA211/APTES/Fe-MoO3/ITO), electrochemical studies were performed. This biosensor exhibited a linear range of 2ng/ml to 32ng/ml, with the sensitivity of 0.01462mAml/ng, and limit of detection was 0.157 ng/ml. This miniaturized electrochemical setup is simple, portable and is economical.