PHYTOCHEMICAL STUDY, ANTIMICROBIAL ANALYSIS AND PARTIAL CHARACTERIZATION OF MEDICINAL PLANT: NYCTANTHES ARBOR-TRISTIS

Abstract

Ayurveda is one of the oldest systems of medicine that uses plants and their extract for treatment and management of various diseased states. Nyctanthes arbor-tristis is well known Indian medicinal plant and the leaf extracts are used at a large extent in Indian traditional medicine. Present investigation deals with the qualitative analysis and quantitative estimation of phytochemicals in leaves of important medicinal Nyctanthes arbor – tristis plant. Qualitative analysis was carried out to identify the different classes of secondary metabolites in various chemical extracts such as water and methanol. Qualitative analysis of the extracts proved the presence of phytochemicals such as Flavonoids, Carbohydrate, Terpenoid, Phenols, Saponins, Tannins, Steroids and Glycosides. As per quantitative estimation we found that the Protein (19.22%) and Tannin (13.1%) are present higher than other compound in plant extracts. Phytochemicals like Flavonoid, Glycoside, Tannic acid, have been reported for significant hepatoprotective, antiviral, antifungal, antipyretic, antimalarial, antibacterial, anti-inflammatory and antioxidant activities. Herein we describe the synthesis of Fe and Cu nanoparticles using plant extract, which offers an efficient, inexpensive, and environmentally friendly method to produce nanoparticles and was characterized by Scanning electron microscope (SEM). The prepared nanoparticles were found to have better antibacterial and antifungal activity than the sample (water and methanol) leaf extract. Nyctanthes leaf extract were studied to determine the quantitative composition of amino acids using Amino Acid Analyzer. Also fatty acid quantitative composition was determined by Gas Chromatography (GC). The aqueous and methanol leaf extract were studied using TLC. Saponins were extracted from methanol leaf extract and were studied and quantified using HPLC.