IN SILICO MOLECULAR INTERACTION STUDIES OF SHRIMP PROTEIN, PMAV WITH WSSV RING FINGER DOMAIN

Abstract

Background: White spot disease (WSD) is an overwhelming syndrome of shrimp Penaeusmonodon in which receptor protein of P. monodon interacts with viral envelops protein, and causes commencement of the disease. PmAV is the first and only anti-viral protein found in shrimp. It was isolated from a virus unaffected shrimp, P. monodon and identified to be up-regulated at the time of viral infection.

Methods: In the present study, we applied molecular modeling and molecular docking to determine the interaction pattern of amino acid residues between PmAV protein and WSSV RING finger domain.

Results and conclusion: Our result showed that PmAV interacts with WSSV RING finger domain and prevents their activity thus inhibiting the establishment of viral infection. Further studies like molecular dynamics simulation of the complex might be applied to open new possibilities for preventing WSD. The quantifiable calculations offer an opportunity for experimental analysis in future as well as provide with an upfront evidence to understand cellular mechanisms underlying the syndrome.