EXTRACTION OF SULFORAPHANE FROM BROCCOLI SEEDS, ITS DETERMINATION BY GAS CHROMATOGRAPHY/MASS SPECTROSCOPY AND ITS INTERACTION WITH HDAC

Abstract

Cancer is the reason for approximately 13 % of death. Other health issues like cardiovascular disorders, lung inflammation, diabetes, kidney failure etc also affecting people’s daily life. People are making their interest for fruits and vegetables rather than medicine because fruits and vegetables contain secondary metabolites which are necessary for good health. In this project I have focused on broccoli extract that is sulforaphane (isothiocyanate). Sulforaphane behaves like antioxidant, antidiabetic, anticarcinogen, anti inflammatory substance etc. I extracted sulforaphane from Broccoli seeds by using liquid liquid phase extraction method which follows crude preparation and it is determined by GC/MS technique.GC/MS does Sulforaphane’s 2D and 3D structure is formed by ACD/3D Chem sketch software. In this project interaction of Sulforaphane and HDAC1 is shown by Docking. By Docking amino acids which are interacting with sulforaphane are characterized and free energies which are reqired its interaction is calculated.