SCREENING OF PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF SARGASSUM SWARTZII AND EVALUATION OF HERBAL PRODUCT FORMULATION

Abstract

Phytochemical analysis and antimicrobial activity of different solvent extracts of Sargassum swartzii were investigated. Sargassum swartzii, a genus of brown algae possesses several therapeutic activities like anti-inflammatory, antioxidant etc. We have isolated different bacterial and fungal strains from soil landfill. The bacterial strains were characterized using biochemical tests and gram staining method while fungal strains were identified on the basis of morphological characteristics. Phytochemical screening and antimicrobial activity of different extracts of brown algae were tested against various bacterial and fungal strains like Aspergillus niger, Aspergillus candidus, Penicillium species etc. using the disc diffusion assay. Qualitative phytochemical analysis of the brown algae extract revealed the presence of flavonoids, saponins, tannin, phenolic, steroid, terpenoid, and phytosterols. The quantitative analysis of solvent extracts of brown algae has been screened. We have checked the antimicrobial activity of brown algae, extracted from different solvent against different pathogenic bacterial and fungal strains. Out of which cow urine extract and benzene extract was found to be very effective against majority of bacterial strains and fungal strains. Minimum inhibitory concentration was also calculated for different solvent extract of brown algae. To confirm the occurrence of the bio active compounds in the crude extracts of Sargassum swartzii thin-layer chromatography (TLC) analysis was performed. The findings of this study showed Sargassum swartzii extracts had significant therapeutic potential and could be introduced for the preparation of novel functional ingredients in cosmeceuticals. In order to identify the cosmetic potential of Sargassum swartzii physical parameters were evaluated. Therefore, further research studies are needed to exploit its maximum therapeutic potential in the field of cosmetic industry for unique and fruitful application.