FABRICATION OF SUBSTRATE IN THE DEVELOPMENT OF A DNA MICROARRAY FOR DIAGNOSTIC APPLICATIONS

Abstract

High throughput techniques (HT) are the need of the hour as a large amount of data needs to be generated for diagnosis or treatment of a disease. DNA microarrays have been devised to provide with this task. But the problem is that the equipment is too bulky and expensive to be owned by small scale industries. This work aims to address the same issue and fabricate substrates to immobilize the probes specific for pathogens or biomarkers of a specific disease. The probes were covalently attached to the substrate, which would be chemically modified to be amenable for attachment, and then hybridized with the target sequence to know the correctness of the system. The efficiency of the system checked by fluorescence images, FTIR data, and surface graphs.