SYNTHESIS AND CHARACTERIZATION OF COPPER OXIDE NANOWIRES FOR APPLICATION IN ELECTROCHEMICAL BIOSENSING

Abstract

In recent years, Nanotechnology has advanced in the field of Science and technology. Because of small size and unique architecture, all the properties of nano materials (physical, chemical or electronic properties) differ from those of bulk material. These materials can be used for the construction of sensing devices, predominately electrochemical sensing devices. Electrochemical sensing find its application for monitoring glucose levels, cancer cells, cholesterol, cardiac biomarker detection and infectious diseases. We report the synthesis of CuO (copper oxide) by a simple, low cost, wet chemical route. Uniform nanowires are grown on Copper foil by oxidising copper. We have reported the growth mechanism for better understanding of optical parameters, that are responsible for controlled growth through a self assembly method. The structural properties, morphology and composition of as synthesized material were explored by X-Ray Diffraction (XRD), Scanning electron microscopy (SEM), and Infrared Spectroscopy (FTIR)respectively. The result obtained, show the formation of highly crystalline metal oxide nanoparticles. Also the studies related to development of biosensor used for cardiac biomarker detection by APTES modified CuO ( APTES/CuO ) is done. ITO electrodes (indium tin oxide) were used to deposit APTES/CuO nanoparticles through electrophoretic deposition (EPD) technique and further cardiac biomarker anti-cTnI was used for electrochemical studies.The electrodes of APTES/CuO/ ITO and anti-cTnI/APTES/CuO/ITO were characterised using FTIR, Cyclic voltammetry (CV) analysis, scan rate, and Electrochemical impedance spectroscopy (EIS). The immunosensor showed superior sensing properties, with wide linear range and high sensitivity.