INFLUENCE OF VARIOUS CARBON AND NITROGEN SOURCE ON BIOMASS AND LIPID PRODUCTIVITY OF CHLORELLA MINUTISS

Abstract

Green fuels are getting greater attention day by day due to increase in energy demands and environmental problems. In the current study, Chlorella minutissima and Scenedesmus sp. were cultivated in BBM medium. The impact of various nitrogen (Sodium Nitrate, Potassium Nitrate, Yeast Extract, Glycine and Urea) and natural carbon (Glucose, Glycerol, Fructose, Maltose, Starch, Sodium acetic acid derivation and Sucrose) sources were broke down for development and lipid collection on these green growth. The most noteworthy biomass development in Chlorella minutissima and Scenedesmus sp. in nitrogen sources was found in Urea (2577.27 mg/L), (1201.3 mg/L) respectively while in natural carbon sources, the biomass development and profitability for Chlorella minutissima was discovered greatest in Fructose (1268.58 mg/L) and for Scenedesmus was observed to be in Starch (1151.33 mg/L). The lipid content was analyzed utilizing Folch strategy by changing dissolvable framework and Chloroform: Methanol (2:1) was seen demonstrating best outcomes and was utilized for extraction. Maximum lipid was found in Potassium nitrate nitrogen source (50.08%) for Chlorella minutissima and for Scenedesmus sp Urea as nitrogen source (79.05 %). Among organic carbon sources, the maximum lipid yield for Chlorella minutissima (36.79% and lipid productivity 2577.27mg/L/day were found in case of Glucose) and for Scenedesmus sp. (27.4 % and lipid productivity 1690.18 mg/L/d were found in Maltose). Further it has been observed both the algae contain Fatty acid from C:16 to C:18 which are essential for biodiesel production. Along with lipid extraction harvesting of biomass is also an important factor which will indicate higher biomass means higher lipid content and it was seen Centrifugation is the best method among various methods tested. Various other analyses done were Chlorophyll estimation, Protein estimation and Carbohydrate estimation to test their presence and quantify it.