AN INVESTIGATION ON RELEASE OF DNA/ CATIONIC LIGAND NANO-COMPLEX FOR GENE DELIVERY APPLICATION

Abstract

The present study focuses on the use of non-viral vectors for the gene delivery applications. Various non-viral vectors are available but lipids and polymers are extensively studied because of theie many advantages. In this investigation we have used G1 & G2 PAMAM dendrimer for the compaction of the DNA and the complex formed is checked by the gel condensation and the Atomic Force Microscopy ( AFM imaging). Gel release assays were conducted and tranfection of the DNA with the help of dendrimer as cationic ligand is checked by transfecting the nanocpmplex in to the CHO-K1 cell line and observing the results under the fluorescent microscope. Flow cytometry is performed to get the quantification o f the transfected cells while by fluorescent microscope helps us know about the qualitative analysis of the tranfection. With its help we are able to see very little transfection into to the by G1 PAMAM dendrimer.