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DC Field | Value | Language |
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dc.contributor.author | DUBEY, DIVYA RANI | - |
dc.date.accessioned | 2016-10-04T05:01:03Z | - |
dc.date.available | 2016-10-04T05:01:03Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.uri | http://dspace.dtu.ac.in:8080/jspui/handle/repository/15129 | - |
dc.description.abstract | This dissertation contains results of the studies related to the fabrication of a label free biosensor based on reduced graphene wrapped cysteine (rGO-Cys) self assembled on gold (Au) electrode for the detection of cardiac troponin I (cTnI) using electrochemical and surface plasmon resonance (SPR) techniques. The rGO-Cys were characterized using transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and contact angle (CA) measurements. Further, SPR studies were used to investigate the immobilization process and the binding kinetics of antigen-antibody interactions and it was observed that 11.7ng of anti-cTnI has been immobilized on the surface of Au electrode under optimized conditions. The response studies carried out using electrochemical impedance spectroscopy shows that the fabricated bio-electrode has sensitivity of 0.0642Ω mLng−1cm-2, linear detection range of 1-80 ngmL-1, linear regression coefficient of 0.9956 and lower detection limit of 0.0697 ngmL−1. | en_US |
dc.language.iso | en_US | en_US |
dc.relation.ispartofseries | TD NO.2433; | - |
dc.subject | REDUCE GRAPHENE OXIDE | en_US |
dc.subject | SURFACE PLASMON RESONANCE | en_US |
dc.subject | CYSTEINE | en_US |
dc.subject | SAMs | en_US |
dc.subject | EIS | en_US |
dc.title | SURFACE PLASMON RESONANCE BIOSENSOR BASED ON GRAPHENE WRAPPED CYSTEINE SELF-ASSEMBLY FOR CARDIAC TROPONIN I | en_US |
dc.type | Thesis | en_US |
Appears in Collections: | M.E./M.Tech. Bio Tech |
Files in This Item:
File | Description | Size | Format | |
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DivyaDubey_MTECHThesis.pdf | 3.69 MB | Adobe PDF | View/Open |
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