NANOSTRUCTURED METAL OXIDE DOPED HYDROGEL BASED BIOSENSOR FOR BIOMEDICAL APPLICATION

Abstract

This dissertation conatins results of the studies related to the fabrication of nanostructured metal oxide doped guar gum hydrogel (GGH/Hydrogel) based immunosensing platform for detection of oral cancer biomarker (CYFRA-21-1). First of all we have synthesized nanostructured zirconia through hydrothermal process thereafter it has been functionalized with serine amino acid and GGH has been synthesized through chemical process. Further make a mixed solution of nanostructured zirconia with GGH in 1:1 ratio in ddH2O. The prepared mixture has been further deposited through drop cast method onto pre-hydrolyzed indium oxide (ITO) coated glass electrode. Anti-CYFRA-21-1 has been immobilized through EDC-NHS chemistry and Bovine Serum Albumin (BSA) has been used for blocking of nonspecific binding sites. X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infra-red spectroscopy (FTIR) have been used to investigate the morphological as well as functional characterization of the synthesized material. However, electrochemical studies have been used to characterize fabricated electrodes as well as response studies. The fabricated immunoelectrode (BSA/anti-CYFRA-21-1/Serine/nZrO2/Hydrogel/ITO) exhibit wider linear detection range (2.5 to 80 ng mL-1), remarkable high sensitivity (0.86 μA mg-1 mL cm-2) with shelf life upto 48 days. The electrochemical response studies of fabricated electrode have been further validated through enzyme linked immunosorbent assay (ELISA) on to obtained saliva sample of oral cancer patients.