TECHNICAL DEVELOPMENT AND CHARACTERIZATION IMPEDIMETRIC BIOSENSOR OF GRAPHENE BASED

Abstract

We report a DEN/PMA/AT/G hybrid based impedemetric sensor for a label-free detection of Creactive protein (CRP) in normal human serum. Dendrimer Solution containing EDC/S-NHS, attached to Graphene through a molecular linker 1-pyrenemethylamine. Which offer free terminal carboxyl group for the site specific covalent biomolecular immobilization of protein antibody, CRP, through carbodiimide coupling reaction to form a impedemetric biosensor. The impedemetric biosensor was characterized by frequency response analysis, and current-voltage (I-V) characteristic studies. The bioelectrode exhibited a linear response of CRP detection in the range of 0.01 μg mL-1 to 10 μg mL-1 with a sensitivity of 21.2 Ω cm2 per decade change in normal human serum.