PHYTO-STEROL ESTIMATION AND GREEN SYNTHESIS OF SILVER NANO-PARTICLE USING LEAF EXTRACT OF PLUMBAGO ZEYLANICA

Abstract

Plants consists of various chemical compounds which have therapeutic value and help to cure various disease including cancer. Since plant products give an advantages of being immune friendly and biocompatible. Plumbago zeylanica L. (family: Plumbaginaceae), commonly called “White leadwort” in English and “Chitrak” in Sanskrit (the most ancient Indian language), is a perennial shrub found in peninsular India and West Bengal and is cultivated in medicinal gardens of India. It has antibacterial, hepato- protective and various other properties but the plant is mostly used for its anticancer property. Till date the plants are taken from the wild for research purpose which poses a threat to its survival in the wild. To prevent the extinction, tissue culture can be used as an important tool for its mass propagation and for production of important phyto-compounds having therapeutic significance which can help in further research on the plant without disturbing it in the wild. The biosynthesis of nanoparticles using the plant extract is also helpful in determining the antibacterial and anti-microbial property of the plant. In this study, effect of different nitrogen source and elicitors were recorded on different accessions of P. zeylanica. Accession number 524441 was found to be potential accession grown on ammonium nitrate among five different accessions and 421418 grown on plant elicitor yeast extract. Also the estimation of Sterols present in the plant which has been cultured on various conditions has to be done to check the variations in the amount of sterols produced estimation using Gas Chromatography. Along with these investigation, all five accessions were also used for their potential of silver nano-particle synthesis.