ESTIMATION OF PLUMBAGIN BY RP-HPLC & FAME ANALYSIS BY GC-MS OF IMPORTANT MEDICINAL PLANT PLUMBAGO ZEYLANICA

Abstract

Plumbago zeylanica is an important medicinal plant used in the cure of various diseases. Due to its various therapeutic values, this plant is being researched for high biomass production as well as achievement of maximum yield of bioactive compounds. In the present study, effect of different media and carbon source on in vitro shoot regeneration of five different accessions of Plumbago zeylanica using nodal explants was investigated. Among the three types of carbon sources that were employed in the present study, sucrose proved to be a better choice for multiple shoot regeneration followed by fructose and glucose in five different accessions of P. zeylanica. MS medium provided maximum growth among four different media tested i.e. MS, White, B5 and Nitsch. Quantitative analysis of important bio-active compound plumbagin was performed using standard protocol by reverse phase high performance liquid chromatography of methanolic extract of roots of accession number 524441 which was found a potential accession in a previous study of plant elicitors. Elicitors enhanced plumbagin concentration three times in comparison with the reference. In addition to these three studies, shoots of this plant was also used for fatty acid methyl ester (FAME) profiling. FAME analysis concluded that this plant is rich in C14, C15, C16 and C18 fatty acids. Maximum percentage of fatty acids is found in accession number 421418. Accession number 524441 showed presence of each fatty acids.