ESTIMATION OF ASIATICOSIDE BY RP-HPLC & FAME ANALYSIS BY GC-MS OF IMPORTANT MEDICINAL PLANT CENTELLA ASIATICA

Abstract

The use of carbon and nitrogen in nutrient media is essential for growth and helps identify highly productive media for Centella asiatica (L.). It is used in traditional medicine for the treatment of various ailments. It contains various secondary metabolites which can be enhanced by the use of elicitors. Present study deals with the quantitative analysis of ethanolic extract of stem and leaf of this plant. An efficient and reproducible protocol was developed for micro propagation and enhances secondary metabolite production using explants of Centella asiatica. The combination of BAP, along with different concentration of sugars, nitrogen source and elicitors were used for this purpose. In between sucrose and fructose, sucrose containing media gives the better result than fructose. Among the nitrogen sources used ammonium nitrate shows best growth and malt extract shows best growth among the three different elicitors. Asiaticoside is one of the important phytocompounds that helps in the treatment of jaundice, missiles, hepatitis, small pox and rheumatism. Due to the importance of asiaticoside there is a need to micro propagate this plant so that maximum yield of asiaticoside can be achieved. Quantitative analysis of asiaticoside was performed using standard protocol by reverse phase high performance liquid chromatography. Fatty acids are of great significance when it comes to food nutrition evaluation, pharmacology and disease diagnosing. Saturated fatty acids helps to reduce cardiovascular risks and improving liver, lungs and brain health. Unsaturated (mono and polyunsaturated) fatty acids are used for declining heart disease and inflammation and increasing the immunity. Due to this medicinal importance, the analyses of fatty acids from five different accession of Centella asiatica were observed.