CORRELATION OF MAST CELL IN GASTRIC CANCER, INSIGHT STUDY OF HRH4 RECEPTOR BY NGS TECHNOLOGIES

Abstract

Gastric cancer is one of the most prevalent type of cancer and is the second most cancer to cause deaths annually. Although there is no perfect treatment of any type of cancer in the world, yet if diagnosed at early stage, can be controlled by surgery, chemotherapy, radiation therapy, or combinations of all which is known as multimodality therapy. We have used RNA seq (from dnanexus.com) of 10 gastric cancer patient and compare them to healthy individuals by keeping reference of already sequenced human genome from UCSC genome browser. We use softwares like BOWTIE, TopHat, Cufflink package and CummeRbund package in this pipeline to analyze the data. We have analyzed 10 RNA seq which are sequenced by Next Generation Sequencing by comparing them to normal data and find that the expression level of HRH4 is decreased by almost twice fold. Role of HRH4 in colorectal cancer, breast cancer and gastric cancer was also previously believed and is yet to be confirmed but our study is step finding towards the association. As previously reported HRH4 is linked to tumor progression in colorectal cancer but our results also clearly shows there is down-regulation of HRH4 in gastric malignancies by almost double fold in both the isoforms of HRH4. Although this work is yet to be validated, so that it can also use as marker in gastric cancer diagnosis.