ROOT ENHANCEMENT IN CHLOROPHYTUM BORIVILIANUM USING SOLID AND LIQUID CULTURE MEDIA

Abstract

Chlorophytum borivilianum has been known for the medicinal uses of its roots. Roots contain many constituents which can helps in curing diseases like cancer, male sterility, diabetes, physical weakness, post natal problems etc. but the species is endangered due to lack of propagating conditions in nature. Its roots are valuable in pharmaceutical industries as well as it can also leads to economic growth because to its high demand in the world market. Plant tissue culture is the biotechnological area that can promise its large scale propagation and helps to meet the ever growing demand. We have tried full strength MS media with different PGRs in both solid and liquid media. Activated charcoal was used as a supplement in the media. It has found that full strength liquid MS media with charcoal added gives out the best results as the rooting media in a short time period as compared to other media composition. The liquid media with 250 mg/l activated charcoal containing 0.8 mg/l NAA and 1 mg/l IAA shows the best result for rooting. While in shooting MS media containing 3 mg/l BAP without AC gives the best result. However solid media containing AC show no appreciable result.