PROTEIN PROFILING AND IN SILICO ANALYSIS OF PPI NETWORK IN MENINGITIS CSF SAMPLES

Abstract

Meningitis is a chronic neurodegenerative disorder in which inflammation of meninges occurs that results in leakage of CSF. Inflammation in meninges, which is the protective covering of the brain and spinal cord elicit the meningitis. Meningitis could be life threatening because of the inflammation of the meninges. Some common causes of meningitis include virus, fungi, bacteria, and parasites. In bacterial meningitis, inflammation of the meninges affecting the arachnoid, subarachnoid, and pia matter space that happens in response to bacterial products. Bacterial meningitis is also originated to be contagious. The mode of action of bacterial meningitis is direct contact with the respiratory secretion as well as throat and oral secretion of an infected person, such as coughing, sneezing etc. Most causative agents found to be Haemophilus influenza, Streptococcus pneumonia, and Neisseria meningitides. Children under the age group of five years get affected by this disease mainly caused by Haemophilus influenzae type b. Etiopathology of bacterial meningitis have been altered by vaccination against following bacteria namely Neisseria meningitides, Streptococcus pneumoniae, and Haemophilus influenzae type b. Vaccines have an essential role in prevention of bacterial meningitis. Vaccines against Streptococcus pneumonia, Neisseria meningitidis, and Haemophilus influenzae are available at present, but each vaccine is specific for each bacterium and limited to some of the serotypes of each bacterium. For example, vaccines are currently available to inhibit Haemophilus influenzae infections due to serotype b but that could not be used for that infection which occurs due to other serotypes. HSPs are conserved proteins found in animals and others, acts as molecular chaperon that gets activated in stress condition and helps in correct folding of proteins. However in case of meningitis, inflammation in meninges ascertains the improper functioning of HSPs. Although in this project I have tried to find out the therapeutic role of two biomolecules i.e., Baicalin and Quercetin which could be used for the cure of meningitis. However, Meropenem is already approved by FDA in 1996 for treating bacterial meningitis. I have taken bexA, ctrA, HPD and SodC that are involved in meningitis and for curing this disease it was tried to retrieve the data required to validate the effectiveness of Quercetin and Baicalin as drug in treatment of meningitis