IDENTIFICATION OF ACTIVE COMPONENTS DERIVED FROM NK RESISTANT CELL LINR RESPONSIBLE FOR NK CELL MODULATION

Abstract

1. ABSTRACT Natural killer cells which have the cytolytic activities against target cells are the white blood cells of the innate immune system, originally identified in 1975. Their classification as lymphocytes depends on their etiology, expression of lymphoid markers, and their genesis from the lymphoid progenitor cell within the bone marrow. NK cells vary from phagocyte like macrophages and granulocytes which completely depend on conserved pattern recognition sequences present on receptors as toll like receptors. Tumors develop numerous mechanisms through which they elude NK cell attack. The NK cells show great role in host defense against virus infected and tumor cells. The interactions between the known factors such as galectin-3, CD63(AD1 antigen), gp70 and p30 present on the surface membrane of p815 tumor with their inhibitory receptors Ly49A were potentially addressable by computational approaches and which could further help to develop NK based cancer therapeutic strategies. A set of proteins were obtained upon extraction with tris-EDTA from plasma membrane of p815 mastocytoma which upon fractionation through amicons provided with proteins between 30 KDa-100KDa. These fractions were then analysed and bound to splenic NK cells to examine the modulations. The sensitivity and resistance of various tumor cell lines to NK cells might open the gateways for NK cell-based immunotherapy including intervention with NK cell activation and inhibition induction as well as NK cell function modulation which might be of inhibition and recognition in NK cell receptor and modulation of the co-stimulation, susceptibility to apoptosis or killing.