PRODUCTION AND CHARACTERIZATION OF RECOMBINANT CELLULOLYTIC RNZYMES

Abstract

Xyloglucan is a hemicellulose polysaccharide mainly found in cell wall of plants. It consists of a backbone similar to cellulose with xylan decoration of β-(1,4)-D-xylanopyranosyl residues. In this study, two previously isolated recombinant strains viz. Xylo and CBM were produced by fermentation wherein Xylo is a recombinant cellulolytic carbohydrate active enzymes (CAZymes) xyloglucan specific endo-1,4-glucanases having molecular mass of 80 kDa which degrades tamarind xyloglucan while CBM is a carbohydrate binding module (CBM46-3) having molecular mass of 25 kDa. Both were purified by Ni-NTA column and their expression, activity and protein content was measured which shows that Xylo is having xyloglucan decomposing activity (to glucose) and can be employed in producing biofuels using lignocellulosic agricultural and industrial waste. Though CBM does not have any xyloglucan degrading activity but have optimal role in increasing local carbohydrate concentrations, binds to insoluble polysaccharides and helps in disruption or modification of carbohydrate structures.