PROCEDURE TO ENHANCE CELLULAR VIABILITY OF BLOOD AND ITS COMPONENTS TO IMPROVE ITS SHELF LIFE

Abstract

1. ABSTRACT Transfusion of blood is important in medical regimen, so for a smooth process a decent inventory should be maintained. Scientific community has given a huge attention towards storage of blood and its components but still there is scope for further research. RBCs and Platelets are the two components of blood majorly used in transfusion, so keeping this in mind efforts have been made to improve the quality of cells under storage conditions. Studies have suggested that Carica papaya leaf extract stabilizes erythrocytes membrane and have the potential to increase RBC and platelet count. Knowing the medicinal value of Carica papaya and its association with diseases like dengue made it the best neutraceutical for the purpose. Promising components were obtained in the GC-MS analysis of Carica papaya leaf extracted in different solvent system namely, Hexane, Acetone, Ethanol and Water, which were further used in different concentrations in blood and platelet to access the quality of cells under storage condition. A few components such as vitamins, tannins, flavonoids, alkaloids, antifungal agents, anti bacterial, oleic acid etc were identified, which could help in blood and platelet for quality improvement. Another approach to increase the quality of platelet during storage condition can be done by adding a specific growth factor. Platelets are generated from megakaryocytic cells in bone marrow using a number of growth factors, but HPCs can easily be directed into megakaryocytic lineage using a single growth factor, Thrombopoietin. So to improve cellular viability of platelet by preventing programmed cell death i.e. apoptosis, an in-silico mimetic for Thrombopoietin was designed. Through the screening of combinatorial library of peptide mimetic, efficient mimetic is reported with the results of docking and physicochemical properties. Mimetic is found to be showing good docking result with an Etotal of -409.66. Resulting peptide mimetic is found stable and has nature similar to TPO. Further Ex-vivo study of designed mimetic with TPOR can confirm its role in increasing the shelf life of platelet. KEYWORDS: Blood, RBC, Platelet, Carica papaya, Shelf life, Storage lesions, TPO, TPOR,