Bio-inspired synthesis of 45S5 bioactive glass using CT-DNA/GELATIN as template

Abstract

Bioactive glass samples (BIS-BG-D/BIS-BG-G) were synthesized by bioinspired rout using CT-DNA/gelatin as template. Additionally, the bioactive glass named SG-BG-G was also synthesized by sol-gel rout using gelatin as template. The biomaterials were characterized by FTIR, SEM, EDS and XRD. SEM micrographs indicated mesoporous microstructure of BIS-BG-D and XRD analysis confirmed the glass samples which are prepared by bioinspired rout are partially crystalline, whereas the glass sample synthesized by sol-gel rout is amorphous in nature. A few characterization tests like density measurement, swelling tests and in vitro degradation studies were also carried out on all bioactive glass samples prepared. Further glass samples are subjected to in vitro bioactivity test by immersing them in simulated body fluid (SBF) at various time intervals. After interaction with SBF the glass samples were subjected to FTIR, XRD, SEM, EDS analysis, the corresponding SBF solutions were tested for pH and also subjected to elemental analysis by ICP-AES. Interestingly, hydroxyl carbonate apatite (HCA) deposition was observed on the surface of the glass pellets after interaction with SBF and the HCA deposition on the glass surface was further confirmed by FTIR, XRD and EDS analysis. Interestingly, the deposition was found to be higher on BIS-BG-D sample due to its mesoporous microstructure. Hence, further experiments like, pH measurement and elemental analysis of SBF after interaction with the glass and cytotoxicity experiments were carried out only on BIS-BG-D sample. The pH of the SBF solution after interaction with BIS-BG-D was found to decrease from 7 to 8.2 after 7 days and remain constant upto 30 days and the corresponding Ca and P ion analysis showed release of these ions from the glass sample into the SBF solution and indicated the similar trend as observed in the case of pH changes observed. In vitro cytotoxicity experiments carried out on BIS-BG-D glass sample using osteosarcoma cells by following MTT assay method indicated the bio glass sample is of good biocompatibility to serve for third generation scaffold materials.